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Atletico Madrid have received their second coronavirus blow in as many days after striker Moussa Dembele tested positive, following team-mate Joao Felix.
It means that Dembele, along with Felix, is now a doubt for Atletico's Champions League round of 16 first leg clash against Chelsea later this month.
A statement from Atletico read: "Our staff underwent PCR tests yesterday as recommended by La Liga, in addition to the first weekly antigen tests that appear in their protocol, and Moussa Dembelehas had a positive result for Covid-19 in the PCR test.
"The forward remains isolated at home in strict compliance with the recommendations of the health authorities and La Liga protocol."
Atletico now have four players in self-isolation - Yannick Carrasco and Mario Hermoso were confirmed to have Covid-19 four days prior to Felix, who tested positive on Wednesday.
Atletico will definitely be without Dembele for their upcoming La Liga fixtures against Celta Vigo and Granada, but he could be out of quarantine by the time they travel to Levante on February 16,although he will only be cleared to play in that fixture after a passing a negative test.
The current Liga leaders are due to play Levante again on February 20, three days before they welcome Chelsea to Wanda Metropolitano for the first leg of their Champions League encounter.
Dembele is yet to make his debut for Atletico since arriving on loan from Lyon on January 13.
He has signed with Diego Simeone's side until the conclusion of the 2020-21 campaign,with an option for the Spanish club to buy for €33.5 million (£30m/$41m) with a potential€5m(£4.5m/$6m)in bonuses. Atletico Madrid will pay a €1.5m loan fee as part of the deal as well.